b4 gsl i b 4 vector laboratories  (Vector Laboratories)

Journal: Frontiers in Neuroscience

Article Title: TIMP3 promotes the maintenance of neural stem-progenitor cells in the mouse subventricular zone

doi: 10.3389/fnins.2023.1149603

Figure Lengend Snippet: Timp2 , Timp3 , and Timp4 are highly expressed in slowly dividing NPCs. (A) The scheme for isolating rapidly dividing and slowly dividing NPCs. H2B-GFP was transiently induced at E9.5 and the LGE was dissected at E17.5. NPCs were defined as cells positive for NPC marker CD133, negative for neuronal marker CD24, and negative for endothelial marker Isolectin B 4 . (B) Representative flow cytometric histogram of H2B-GFP fluorescence intensity. Rapidly dividing NPCs (bottom 10% of NPCs for H2B-GFP intensity) and slowly dividing NPCs (top 10% of NPCs for H2B-GFP intensity) were collected. (C) Quantitative RT-PCR analysis of Timp2 , Timp3 , and Timp4 mRNAs. Data are means ± SEM ( n = 3 independent experiments). * p < 0.05 by two-tailed paired t test.

Article Snippet: Dissociated cells were incubated first for 15 min on ice in 0.2% bovine serum albumin (BSA)/phosphate-buffered saline (PBS) with primary antibodies—PE/Cy7 anti-CD133 (1:100, BioLegend Cat# 141210, RRID:AB_2564069), APC anti-CD24 (1,100, BioLegend Cat# 101814, RRID:AB_439716), and anti-Isolectin B 4 (biotin conjugate) (1,1,000, Sigma-Aldrich Cat# L2140, RRID:AB_2313663)—and then for 5 min on ice in 0.2% BSA/PBS with Streptavidin PE (1,500, eBioscience Cat# 12-4317-87).

Techniques: Marker, Fluorescence, Quantitative RT-PCR, Two Tailed Test

Journal: The Journal of comparative neurology

Article Title: Characterization of the expression of gastrin-releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans

doi: 10.1002/cne.25376

Figure Lengend Snippet: Double fluorescence of the GRP (green) and superficial layers markers (magenta) of Sp5C and cervical spinal dorsal horn in adult macaque monkeys. Many GRP-ir fibers were colocalized with the marker for peptidergic neurons CGRP (a–c) and substance P (d–f) in the Sp5C. GRP-ir fibers were not colocalized with the marker for nonpeptidergic neurons IB 4 in the Sp5C (g–i). Most GRP-ir fibers were colocalized with CGRP(j–l) and substance P (m–o) in the superficial layers of the cervical spinal cord. GRP-ir fibers were not colocalized with IB 4 (p–r) in the cervical spinal dorsal horn. Bars = 200 μm (i, r)

Article Snippet: Double-fluorescence staining of GRP and biotin-conjugated isolectin B 4 (IB 4 ) (1:1,000; cat. no. 121414; Molecular Probes, Eugene, OR) was also performed.

Techniques: Fluorescence, Marker